Purification of a tumour cell aggregation-promotin factor associated with rat ascites hepatoma cell surface
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چکیده
منابع مشابه
Roles of calcium in aggregation of rat ascites hepatoma cells by cell surface-associated adhesive factor.
Our previous studies have shown that a cell surface-associated adhesive factor (AF), separated from rat ascites hepatoma AH136B cells of a differentiated type and highly purified by chromatography, induces not only aggregation of dissociated AH136B cells or rat ascites hepatoma AH109A cells of an undifferentiated type but also adhesiveness characterized by the development of junctional complexe...
متن کاملAn immunoelectron microscopic study on the distribution of a cell surface-associated adhesive glycoprotein synthesized by rat ascites hepatoma cells.
As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF synthesis by the cells was investigated by means of ultrastructural immunoperoxidase cytochemistry. Its synthesis and localization in vivo were observed in the perinuclear spaces, the ...
متن کاملPurification of thioredoxin from rat Novikoff ascites hepatoma.
Partial purification of a thioredoxin system from Novikoff ascites hepatoma cells has been previously reported (MOORE, E. C. (1967) Biochem. Biophys. Res. Commun. 29, 264-268). Thioredoxin from the same mammalian source has now been purified to electrophoretic homogeneity by ammonium sulfate fractionation, heat treatment, DEAE-cellulose chromatography, and Sephadex chromatography. 1-Dimethylami...
متن کاملhigh level of gst-p mrna in island type of rat ascites hepatoma cell lines
several cell lines (18) of poorly differentiated rat ascites hepatomas which grown intraperitoneally were analyzed with respect to their mrna levels of gst-p and albumin. the expression of albumin was dramatically decreased and became undetectable in all of the examined hepatomas compared with normal rat liver. the gst-p mrna level in the hepatomas was correlated with their ability for formatio...
متن کاملInteraction of rat ascites hepatoma cells with cultured mesothelial cell layers: a model for tumor invasion.
Interactions of rat ascites hepatoma cells with primary cultured layers of rat mesentery-derived cells were studied. The mesentery-derived cells were isolated from rat mesentery and cultured in Eagle's minimum essential medium with a 2-fold concentration of amino acids and vitamins supplemented with 10% calf serum. The primary cultured cells, consisting mainly of mesothelial cells in polygonal ...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1976
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1976.126